In vitro evaluation of probiotic bacteria and yeast growth, pH changes and metabolites produced in a pure culture system using protein base products with various added carbon sources

This study was designed to evaluate the changes number of cells two selected probiotic bacteria, B. longum TISTR2195 and L. plantarum TISTR1465, yeast S. cerevisiae TISTR8656. The microorganisms were cultured in types media, namely selective media protein base model. Selective divided into 4 sub-groups: with 2% glucose added as a positive control (i), inulin (ii), fructo-oligosaccharide (FOS) (iii), without carbon source negative (iv). Protein had chicken egg coconut juice, baker’s potassium chloride 9% FOS or 7.34% whey (iv) verify growth. All probiotics able utilize numbers increased incubation time. However, produced an acidic culture medium significantly. based supported all In addition, acetic acid content highest among short-chain fatty acids (SCFAs) produced. very fast within 24 h. provided peak level at 72 tended decrease SCFAs